BioProcess International Poster Preview: Case Studies of hERG Membrane Protein and Japanese Encephalitis Virus Production Using a New Innovative Moving Bed Bioreactor

Leading up to the BioProcess International Event, we'll be highlighting many of the posters you'll be able to find in the hall at the event taking place October 20-23, 2014 in Boston, MA. If you'd like to join us at the event, as a reader of this blog, when you register to join us and mention code BLOG13JP, you'll save 20% off the standard rate!

Poster: Case Studies of hERG Membrane Protein and Japanese Encephalitis Virus Production Using a New Innovative Moving Bed Bioreactor

Presenter: Lewis Ho,  BioReactor Sciences

About: Human ERG membrane protein has gained great interest as a target for drug discovery. A two fold increased expression of the hERG gene was induced under nutrient limitations. The protein is membrane bound and therefore whole cell recovery from the carriers is required. HEK293 cell was used. Japanese encephalitis virus (JEV) for vaccine has long been the only reliable solution to prevent deadly encephalitis. The virus however is unstable and post-infection conditions for optimal virus production and recovery is critical. We use two case studies to illustrate a new innovative Moving Bed (MB) bioreactor’s unsurpassed versatility and functionality to accommodate specific desired conditions and to accomplish the entire upstream processes in one single bioreactor. The mobility of the moving matrix bed allows for the least amount of volume required for seeding, yielding high seed density resulting in increased cell attachment. Similarly, this feature results in high virus infection and DNA transfection efficiency. The MB system facilitates the principle similar to roller bottles intermittently exposing and submersing cells to air and medium to achieve maximum oxygenation with near minimal shear, and also eliminating foaming issues. The moving bed sets a new precedent in performing the mechanism of cell detachment and recovery in place.

Are you interested in presenting your own findings? BioProcess International is accepting poster submissions through September 19.  For more information and how you can submit your poster to be presented in the poster hall, visit our BioProcess International Poster Page.

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What posters can you see in the BioProcess International Exhibit Hall?

Over the past few months, we've been profiling some of the posters that will be featured next week in the BioProcess International Exhibit Hall. Would you like to hear more about any of these posters or discuss them with their presenters? Join us in Boston! As a reader of this blog, when you register to join us and mention priority code  BLOG13JP, you'll save 20% off the standard rate!

Here is a comprehensive list of the posters we have previewed here at the Future of Biopharma Blog:

• - Assessment Of Process Performance And Product Quality In High Performing Fed-Batch Cultures - GE Healthcare
• - Influence of Soy Protein Hydrolysates on Robustness of Cell Culture Experiments - Frieslandcampina Domo
• - TAPBOOST technology: Novel Technology to Enhance the Production of Therapeutic Recombinant Proteins Through Protein Folding System - Boston Strategics Corporation
• - Evaluating the Impact of Controlled Nucleation on Lyophilized Product Attributes and Processing Characteristics in an Aseptic Environment - Lyophilization Technology
• - Use of a Simple Visual Tool to Monitor Commercial-Scale Purification Process Performance on the Manufacturing Floor - Bayer HealthCare
• - Small Scale Protein Purification for Rapid Optimization of Biotherapeutic Protein Production - PerkinElmer
• - Linear Scalability of Virus Production in Integrity® iCELLis® Single-Use, Fixed-Bed Bioreactors From Bench Scale to Industrial Scale - ATMI Life Sciences
• - High Throughput Viscosity Measurement for Assessing Biologics Formulations - FreeSlate
• - Near-universal Similarity Bounds for Bioassays - Precision Bioassay, Inc
• - Differentiation of Protein Particles and Silicone Oil Droplets by Flow-imaging Microscopy (MFI and FlowCAM) and Resonant Mass Measurement (Archimedes)- Coriolis Pharma Research GmbH
• - Evaluation of the Cedex Bio and HT Metabolite Analyzers for Cell Culture Process Monitoring - Genzyme
• - Using Helium Integrity Tester to Increase Assurance of Sterility of Single-use Assemblies - ATMI Life Sciences
• - The Importance of Thresholding in Imaging Analysis of Protein Aggregates - Fluid Imaging Technologies
• - Enhanced 2D Electrophoresis and Western Blotting Workflow for Reliable Evaluations of Anti-HCP Antibodies - BioRad

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BioProcess International Poster Feature: EtoxiClear™: A New Adsorbent for the Efficient Removal of Endotoxin From Biopharmaceuticals

Last week, we put out a Call for Posters so that you could share your research and findings with your scientific community at the BioProcess International Conference this coming September 16-19, 2013, in Boston.  This week, we'll be looking at the two winning posters that were presented at the 2012 BioProcess International Event.  Monday, we featured Characterization of a Biomanufacturing Fermentation Process Using a New DOE Approach: Definitive Screening Designs.  Today, we feature the other winner of the BPI International poster contest.

EtoxiClear™: A New Adsorbent for the Efficient Removal of Endotoxin From Biopharmaceuticals

by Ben Galarza, Jason Shipp, Buzz Lobbezoo, Jennie Walden and Steve Burton

ProMetic BioSciences Ltd

About the poster:

Endotoxin or lipopolysaccharides (LPS) are highly toxic components of the cell wall of Gram-negative bacteria and are often present in significant amounts in bacterial cell culture expression systems such as E. coli. A number of methods have been adopted for the removal of endotoxin based on adsorption, in particular ion-exchange chromatography. Although downstream processing can significantly reduce endotoxin levels in the product, efficient and cost-effective removal of residual endotoxin from biopharmaceutical preparations remains a challenge.

This technical poster addresses the issues of removal of endotoxin from biological preparations. Specific reference will be made to a new synthetic ligand affinity adsorbent, EtoxiClear™, which exhibits high affinity for endotoxin, low protein binding and can be depyrogenated using sodium hydroxide. The bi-dentate ligand, attached to PBL’s proprietary base matrix – PuraBead®, binds in a spatially selective and optimal manner to the LPS molecule with a dynamic binding capacity for endotoxin in excess of 1,000,000 EU/mL of adsorbent (5 minute residence time). A number of biomolecules with different isoelectric points were used to demonstrate efficient recovery and clearance of residual endotoxin across the pH range. Protein recoveries in excess of 95% are achievable with endotoxin clearance to below 0.1 EU/mg protein.

Download the poster here.

If you'd like to join the Poster Hall at this year's BioProcess International, find out more at the webpage!

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