Tuesday, August 27, 2013

BioProcess International Poster Preview: Differentiation of Protein Particles and Silicone Oil Droplets by Flow-imaging Microscopy (MFI and FlowCAM) and Resonant Mass Measurement (Archimedes)

Leading up to the BioProcess International Event, we'll be highlighting many of the posters you'll be able to find in the hall at the event taking place September 16-19, 2013 in Boston, MA. If you'd like to join us at the event, as a reader of this blog, when you register to join us and mention code BLOG13JP, you'll save 20% off the standard rate!

Poster: Differentiation of Protein Particles and Silicone Oil Droplets by Flow-imaging Microscopy (MFI and FlowCAM) and Resonant Mass Measurement (Archimedes)

Presenter: Andrea Hawe,
Coriolis Pharma Research GmbH

About the poster:We comparatively evaluated flow-imaging microscopy and the recently introduced technique of resonant mass measurement (Archimedes, RMM) as orthogonal methods for the quantitative differentiation of silicone oil droplets and protein particles in the submicron and micron size range. Representative samples of artificially generated silicone oil droplets and protein particles were prepared and analyzed both separately and in defined mixtures by the different instruments (MFI4100 and MFI5200, Protein Simple; FlowCAM VS1 and FlowCAM PV, Fluid Imaging). An accurate differentiation of silicone oil droplets and protein particles could be achieved by all flow-imaging microscopy systems in the micron size-range. Clearly lower standard deviations (higher precision), were obtained by MFI5200 and FlowCAM PV due to the higher sampling efficiency, as compared to MFI4100 and FlowCAM VS1. The customized filter (MFI4100) was more reproducible and enabled reliable discrimination for more extreme mixing ratios. RMM showed highly accurate discrimination form about 0.5 to 2 ┬Ám independent of the ratio between silicone oil droplets and protein particles, provided that sufficient particles were counted. We recommend combining flow imaging microscopy and resonant mass measurement for a comprehensive analysis of biotherapeutics potentially containing silicone oil droplets and protein particles in the submicron and micron size range.


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