Friday, November 29, 2013

Antibody Engineering Poster: Preparation and Characterization of Recombinant Antibodies from Insect Cells

Leading up to the Antibody Engineering Event, we'll be sharing the posters that will be featured in the Exhibit Hall.  Antibody Engineering & Therapeutics will take place December 8-12, 2013 in Huntington Beach, California.  For more information the program, download the agenda.  If you'd like to join us, as a reader of this blog, when you register to join us and mention code XD13172BLOGJP, you'll save 20% off the standard rate!

Featured Poster: Preparation and Characterization of Recombinant Antibodies from Insect Cells

Featured Presenter: Rachel Cheng, Academia Sinica

About the poster: Human or humanized monoclonal antibodies and related products are the fastest growing class of human therapeutics. More than 40 antibodies have been approved for use in various indications such as cancer and inflammatory diseases. Most of antibodies using for therapeutics are currently produced using CHO cells. However, the antibodies produced from CHO cells would contain core alpha-1,6-fucose, which has been shown to significantly decrease the affinity of Fc to FcγIIIa receptor that correlates with the antibody’s ADCC function and anticancer efficacy in vivo. To produce antibodies without core alpha-1,6-fucose, we explored the use of insect cells for antibody production followed with glycosidase digestion of antibodies. Insect cell expression system has been widely used for recombinant protein preparation. The N-glycan of insect-produced proteins mainly composed of pauci-mannose. Herein, we reported successful preparation of full-length antibodies from insect cells. The N-glycan of produced antibodies was confirmed to contain fucosylated Man3GlcNAc2 and the core-fucose can be removed by combination of glycosidases.

Do you have new research to share with your industry colleagues? Your poster can be featured in the Antibody Engineering Exhibit hall as well! Find out how here.  The deadline for poster submissions is November 1.

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