Tuesday, July 15, 2014

Cell Line Development: MicroRNAs in a Commercial Process

A few weeks ago, we wrote about an interview with Dr. Niall Barron, who’ll be speaking at this year’s Cell Line Development & Engineering conference, in which he explained why researchers should be paying attention to MicroRNAs.  Given that an estimated 50% to 70% of all protein encoding genes are under the control of MircoRNAs themselves, it’s clear that these molecules should be peaking the interest of researchers.  But how can they be leveraged in the commercial sense?  We go back to Dr. Barron for the answer.

How might they be implemented by industry in a commercial process?

Dr. Barron: So, as I mentioned previously, there are two main approaches. Ideally, an industry would use this technology before going through regulatory submission. So, with a new cell line making a particular product of interest, a cell line would be manipulated stably by using something like the technology I just described. For instance, over expression sponge that would target the microRNA of interest, or indeed, over expressing a short hairpin that will mimic the microRNA of interest if over expression is desired. So, that forms a stable cell line that can act as the parent, into which the product gene or the transgene is placed and that would go through the normal regulatory process.

The alternative in existing process where full regulatory submission is not desirable would be to add either mimics or inhibitors, which are short molecules that are typically complexed with some kind of transfection reagent and that can be fed directly into the culture medium where it is transported across the outer cell membrane to mediate its effect directly in the process.

You can hear the complete interview with Dr. Barron here, or you can download the transcript included in our brochure here.

Get the latest from Dr. Barron and other industry experts at this year’s Cell Line Development and Engineering conference, September 8-10, Berkeley, CA.  Now, SAVE 20% off the standard rate*.  Register here and use code XB14189BLOG.

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