Wednesday, June 11, 2014

Timeline Reduction in Cell Line Development

What are the most important attributes in developing recombinant cell lines?  For Alison Porter, Head of Mammalian Cell Culture R&D for FUJIFILM Diosynth Biotechnology, the list includes high titer, growth properties that are suitable for a manufacturing process and production of product with the correct characteristics.  The importance of these qualities can’t be overstated, however, achieving them in an efficient way is a priority as well.   We went ahead and got Alison’s take on how to improve some of these characteristics with a reduced timeline in mind.  Check out what she had to say:

What can you target if you want to reduce timelines whilst improving some of these desirable attributes?

Alison: To answer this, I’m going to concentrate on the idea of improving titer whilst reducing timelines. Probably, increasing titer is the most common goal when looking to improve desirable attributes further. When people are thinking about increasing titer over the past 20 to 25 years, the obvious and main contributor that comes to mind is, of course, design of media and feeds in particular.

But there are other contributors from the cell line development stage that can play a role in improving titer whilst reducing timelines. The host cell line, vector and the cell line development process itself. If, for example, we think about the cell line development process, introduction of improved screens can allow earlier selection of the best cell lines. By improved screens, I’m thinking about things like multi-well plate systems, which are operated in suspension mode and have feeds applied, and micro bioreactor systems. So, you hopefully end up using these as being both better at identifying cell lines capable of high expression and identifying them earlier on.

Cell Line Development Time Line Reduction
September 8-10, Berkeley, CA
To think about host cell lines, work can be done to develop new host cell lines with increased expression capability and you can also improve them with regards to growth characteristics - and that will also potentially help reduce timelines.

At FUJIFILM, we’ve actually been looking at all three of these areas recently – host cell lines, vector and cell line development process. Looking to further improve our cell line development capabilities.

To check out the full interview with Alison Porter, download the Cell Line Development and Engineering brochure.

We’ll have more from Alison at this year’s Cell Line Development & Engineering conference.  Join us September 8-10 in Berkeley, CA.  Save 20%* off the standard rate when you register here and use discount code XB14189BLOG.   

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