Chandra Dixit, Researcher, National Biophotonics & Imaging Platform of Ireland recently sat down with the Cell Based Assays and Bioanalytical Method Development team to discuss various methods of improving assay sensitivity and signal-to-noise ratios, in the context of his own work in developing high-sensitivity surface plasmon resonance immunoassays and nanoparticle-based imaging platforms.
Here's a excerpt from the podcast:
Could you describe your own work in bioanalytical method development?
Dixit: I am working on developing different platforms, like if you’re working assay development, you might have heard about developing immunoassays for phased plasmon resonance on focal imaging. So, what I’m trying to do is I’m trying to build a bioassay that is highly sensitive. When we are talking about enzyme immunoassays, then I’m trying to improve the sensitivity so that we can go far below the thresholds and that could be used to – that have huge applications in industry and diagnostics. And when I’m talking about SPL immunoassays and imaging platforms, then we certainly can go and make visualization of various particles. So, what I am doing is I’m developing a platform that could detect viruses or bacteria or cellular components down below that threshold that’s in our bodies. So, I will go one by one what exactly I am doing.
First of all, I was working with Bristol-Meyers Squibb. With them, what I did – they have bioprocess technologies going on. They develop different pharmaceutical products. So, they expected me to develop immunoassays that can detect impurities in their bioprocess systems. So, I developed five immunoassays, which are now translated and the technologies are transferred to them. So, in other cases I have developed few Surface Plasmon Resonance assays and currently I’m working on developing different high-sensitivity nanoparticle-based imaging systems, which uses silicone nanoparticle and which is, of course, high dose. These dyes are near infrared dye, which comes into the range of – the fluorescence comes into the range of near infrared region. So, this is all about my work.
To read the entire transcript and listen to the podcast, download it here.
Cell Based Assays and Bioanalytical Method Development event is taking place October 3-5 in Berkeley, CA. For more information on Dixit's presentation and the rest of the program, download the brochure. As a reader of the Future of Biopharma Blog, register for this event with the special Priority Code XP1668Blog, to receive 25% off the standard rate! If you have any questions about the event, please feel free to contact Jennifer Pereira at jpereira@iirusa.com.
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