What is antibody validation?

We recently had a chance to sit down with a few of the Antibody Engineering and Therapeutics speakers to get an inside look into what they're working on and insights into their work.  We start this interview series off with Dr. David Rimm, a Professor of Pathology and Director, Yale Pathology Tissue Services and Yale University School of Medicine.

Today, he starts of by answering the question:
What is antibody validation?

Dr. Rimm's answer:

Antibody validation means different things to different people. Essentially, it means proving that the antibody received from the vendor – as constructed by one’s own lab – actually interacts or binds to the epitope that is allegedly to which the antibody was raised or to the epitope that is interacting with the antibody binding site.

The methods for validation are exactly how validation is done. It is variable and standardization would probably be helpful for determining exactly how validation is done. Some antibody validation is done very superficially and ultimately leads to false results or inaccurate results. While other people validate their antibody using much more extensive techniques, that’s what we discussed at the meeting and these results can be trusted.

Dr. Rimm will be presenting Use and Abuse of Antibodies in Research and the Clinic on Tuesday, December 9.  For more information on this session and the rest of the program, download the agenda. As a reader of this blog, when you register to join us December 8-12, 2014, in Huntington Beach, California and mention code XD14172BLOGJP, you can save 20% off the standard rates.

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